Journal: International Journal of Dentistry
Article Title: Overexpression of the IL‐23/IL‐17A Axis and Their Receptors (IL‐23R and IL‐17RA) in Gingival Tissue of Patients With Periodontitis
doi: 10.1155/ijod/8893504
Figure Lengend Snippet: Correlation between IL‐23/IL‐17A axis molecules and IL‐23R and IL‐17RA receptors with clinical parameters in gingival tissue. The Spearman correlation test was used to analyze the values of periodontal clinical parameters and the concentrations of IL‐23, IL‐17A, IL‐23R, and IL‐17RA. BoP%, percentage of bleeding on probing; CAL, clinical attachment loss; IL, interleukin; PD, probing depth; RBL%, radiographic bone loss, ∗ p ≤ 0.05, ∗∗ p ≤ 0.001.
Article Snippet: The membranes were then incubated with the appropriate primary antibody overnight at 4°C: mouse monoclonal anti‐human IL‐17 (G‐4) IgG2B diluted 1:500 (sc‐374218, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti‐human IL‐23 (c‐3) monoclonal mouse IgG1 diluted 1:500 (sc‐271279, Santa Cruz Biotechnology), anti‐human IL‐17RA (D1Y4C) monoclonal rabbit IgG diluted 1:1000 (12661, Cell Signaling Technology, Danvers MA, USA), anti‐human IL‐23R (A48090) polyclonal rabbit IgG diluted 1:300 (Antibodies.com, Cambridge, UK), and β‐actin (C4) mouse monoclonal IgG1k diluted 1:1000 (sc‐47778, Santa Cruz Biotechnology).
Techniques: