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mouse monoclonal igg1 antibody  (R&D Systems)


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    Structured Review

    R&D Systems mouse monoclonal igg1 antibody
    Mouse Monoclonal Igg1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+human+il/Human%2FPrimate+IL-6+Antibody/pmc13021408-195-4-13
    Average 95 stars, based on 143 article reviews
    mouse monoclonal igg1 antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis
    Article Snippet: Samples were collected and analyzed with on a Sony SH800S Cell Sorter (Sony Biotechnology, USA).

    Immunopeptidomics:

    Article Title: Superantigen presentation by airway smooth muscle to CD4+ T lymphocytes elicits reciprocal proasthmatic changes in airway function.
    Article Snippet: The human ASM cells were obtained from BioWhittaker, Ham’s F-12 medium was purchased from Mediatech, and 10% FBS was obtained from HyClone Laboratories. .. The mouse antihuman monoclonal MHC class II (HLA-DR) neutralizing Ab (clone G46-6) and the anti-CD54 monoclonal blocking Ab were purchased from BD Biosciences, and the mouse monoclonal anti-human IL-13 neutralizing Ab and the human IL-13 ELISA kit were obtained from R&D Systems. .. Alexafluor 568-conjugated highly cross-adsorbed goat anti-mouse Ab was obtained from Molecular Probes/Invitrogen.

    Blocking Assay:

    Article Title: Superantigen presentation by airway smooth muscle to CD4+ T lymphocytes elicits reciprocal proasthmatic changes in airway function.
    Article Snippet: The human ASM cells were obtained from BioWhittaker, Ham’s F-12 medium was purchased from Mediatech, and 10% FBS was obtained from HyClone Laboratories. .. The mouse antihuman monoclonal MHC class II (HLA-DR) neutralizing Ab (clone G46-6) and the anti-CD54 monoclonal blocking Ab were purchased from BD Biosciences, and the mouse monoclonal anti-human IL-13 neutralizing Ab and the human IL-13 ELISA kit were obtained from R&D Systems. .. Alexafluor 568-conjugated highly cross-adsorbed goat anti-mouse Ab was obtained from Molecular Probes/Invitrogen.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Superantigen presentation by airway smooth muscle to CD4+ T lymphocytes elicits reciprocal proasthmatic changes in airway function.
    Article Snippet: The human ASM cells were obtained from BioWhittaker, Ham’s F-12 medium was purchased from Mediatech, and 10% FBS was obtained from HyClone Laboratories. .. The mouse antihuman monoclonal MHC class II (HLA-DR) neutralizing Ab (clone G46-6) and the anti-CD54 monoclonal blocking Ab were purchased from BD Biosciences, and the mouse monoclonal anti-human IL-13 neutralizing Ab and the human IL-13 ELISA kit were obtained from R&D Systems. .. Alexafluor 568-conjugated highly cross-adsorbed goat anti-mouse Ab was obtained from Molecular Probes/Invitrogen.

    Incubation:

    Article Title: Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes
    Article Snippet: They were then incubated with peroxidase-blocking solutions (Dako, Glostrup, Copenhagen, Denmark) for 10 min at room temperature and blocking solution (Life Technologies, Carlsbad, CA, USA) at 4 °C overnight. .. Next, the sections were incubated with rabbit polyclonal anti-human IL-23Ap19 antibody (MBS240347; MyBio Source, San Diego, CA, USA) or mouse monoclonal anti-human IL-27/IL-35 EBI3 antibody (MAB6456; R&D Systems, Minneapolis, MN, USA) at 4 °C overnight. .. Finally, the slides were incubated with goat anti-mouse/rabbit IgG antibodies conjugated to peroxidase-labeled polymer (Dako, Tokyo, Japan) for 30 min. Histochemical visualization was carried out with 3,3-diaminobenzidine (DAB) (Dako).



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    Image Search Results


    IL‐23 in gingival tissue by western blotting. Representative blot of HS ( n = 4) and P ( n = 4) samples. The expression of IL‐23 in gingival tissue was evaluated using the western blotting technique and was expressed as ARU. Representative bands and graphs of IL‐23 (19 kDa) and β‐actin (43 kDa). ARU, area relative units; HS, healthy subjects; MW, molecular weight; P, periodontitis. Medians and the maximum and minimum represent data (Mann–Whitney U statistical analysis).

    Journal: International Journal of Dentistry

    Article Title: Overexpression of the IL‐23/IL‐17A Axis and Their Receptors (IL‐23R and IL‐17RA) in Gingival Tissue of Patients With Periodontitis

    doi: 10.1155/ijod/8893504

    Figure Lengend Snippet: IL‐23 in gingival tissue by western blotting. Representative blot of HS ( n = 4) and P ( n = 4) samples. The expression of IL‐23 in gingival tissue was evaluated using the western blotting technique and was expressed as ARU. Representative bands and graphs of IL‐23 (19 kDa) and β‐actin (43 kDa). ARU, area relative units; HS, healthy subjects; MW, molecular weight; P, periodontitis. Medians and the maximum and minimum represent data (Mann–Whitney U statistical analysis).

    Article Snippet: The membranes were then incubated with the appropriate primary antibody overnight at 4°C: mouse monoclonal anti‐human IL‐17 (G‐4) IgG2B diluted 1:500 (sc‐374218, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti‐human IL‐23 (c‐3) monoclonal mouse IgG1 diluted 1:500 (sc‐271279, Santa Cruz Biotechnology), anti‐human IL‐17RA (D1Y4C) monoclonal rabbit IgG diluted 1:1000 (12661, Cell Signaling Technology, Danvers MA, USA), anti‐human IL‐23R (A48090) polyclonal rabbit IgG diluted 1:300 (Antibodies.com, Cambridge, UK), and β‐actin (C4) mouse monoclonal IgG1k diluted 1:1000 (sc‐47778, Santa Cruz Biotechnology).

    Techniques: Western Blot, Expressing, Molecular Weight, MANN-WHITNEY

    Correlation between IL‐23/IL‐17A axis molecules and IL‐23R and IL‐17RA receptors with clinical parameters in gingival tissue. The Spearman correlation test was used to analyze the values of periodontal clinical parameters and the concentrations of IL‐23, IL‐17A, IL‐23R, and IL‐17RA. BoP%, percentage of bleeding on probing; CAL, clinical attachment loss; IL, interleukin; PD, probing depth; RBL%, radiographic bone loss, ∗ p ≤ 0.05, ∗∗ p ≤ 0.001.

    Journal: International Journal of Dentistry

    Article Title: Overexpression of the IL‐23/IL‐17A Axis and Their Receptors (IL‐23R and IL‐17RA) in Gingival Tissue of Patients With Periodontitis

    doi: 10.1155/ijod/8893504

    Figure Lengend Snippet: Correlation between IL‐23/IL‐17A axis molecules and IL‐23R and IL‐17RA receptors with clinical parameters in gingival tissue. The Spearman correlation test was used to analyze the values of periodontal clinical parameters and the concentrations of IL‐23, IL‐17A, IL‐23R, and IL‐17RA. BoP%, percentage of bleeding on probing; CAL, clinical attachment loss; IL, interleukin; PD, probing depth; RBL%, radiographic bone loss, ∗ p ≤ 0.05, ∗∗ p ≤ 0.001.

    Article Snippet: The membranes were then incubated with the appropriate primary antibody overnight at 4°C: mouse monoclonal anti‐human IL‐17 (G‐4) IgG2B diluted 1:500 (sc‐374218, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti‐human IL‐23 (c‐3) monoclonal mouse IgG1 diluted 1:500 (sc‐271279, Santa Cruz Biotechnology), anti‐human IL‐17RA (D1Y4C) monoclonal rabbit IgG diluted 1:1000 (12661, Cell Signaling Technology, Danvers MA, USA), anti‐human IL‐23R (A48090) polyclonal rabbit IgG diluted 1:300 (Antibodies.com, Cambridge, UK), and β‐actin (C4) mouse monoclonal IgG1k diluted 1:1000 (sc‐47778, Santa Cruz Biotechnology).

    Techniques: